5190-2447, Agilent Bio WCX, NP5, 4.6 x 250 mm, PEEK

The Agilent Bio WCX NP5 Weak Cation-Exchange LC Column separates charged protein and peptide species using a weak cation-exchange stationary phase. A 4.6 × 250 mm bed packed with nonporous 5 µm particles provides short intraparticle diffusion paths for biomolecular charge-variant analysis. PEEK hardware supports use in appropriately configured bio-inert liquid chromatography systems and helps reduce unwanted interaction with metallic flow-path surfaces. Retention depends on analyte charge, mobile-phase pH and ionic strength rather than hydrophobicity or molecular size. Develop a suitable salt or pH gradient to resolve acidic, main and basic variants of a target protein. Filter samples and buffer components, use compatible salts and avoid particulate loading that can raise column pressure. Equilibrate the column thoroughly before injections and track resolution and retention reproducibility with a suitable system-suitability sample. This is a weak cation-exchange analytical column, not a reversed-phase C18 column or a size-exclusion column.