| Brand | HC(2)/TC(2) |
|---|---|
| Carbon Load | 17 % |
| Endcapped | Yes |
| Guard Column | No |
| Hardware | Conventional |
| Inner Diameter (ID) | 4.6 mm |
| LC Platform | HPLC |
| Length | 250 mm |
| Maximum Temperature | 60 °C |
| Molecular Weight Lower Limit | 0 Da |
| Molecular Weight Upper Limit | 3000 Da |
| Particle Size | 5 µm |
| Particle Type | Fully Porous |
| Phase | C18 |
| Pore Size | 170 Å |
| Pressure Rating | 400 bar |
| Separation Mode | Reversed Phase |
| UNSPSC Code | 41115709 |
| pH Range | 2-9 |
Answer:It is a 4.6 mm internal-diameter by 250 mm HPLC column packed with 5 µm C18 particles.
Answer:The column has nominal 170 Å pores and a stated pressure rating of 400 bar.
Answer:No. It is a full-length analytical reversed-phase HPLC column; optional guard protection is separate.
The Agilent HC-C18(2) HPLC column provides reversed-phase separation of suitable small organic molecules using an endcapped octadecylsilane stationary phase. The packed bed consists of 5 µm fully porous particles with a nominal 170 Å pore size, housed in a conventional 4.6 × 250 mm column format. A relatively high carbon loading of approximately 17% contributes to hydrophobic retention characteristics useful in method development and routine liquid chromatography. The column has a 400 bar pressure rating, but actual operating limits must also respect the instrument, mobile phase, temperature and fittings. Choose a sample solvent compatible with the mobile phase to reduce injection-related peak distortion. Filter particulate-rich samples and use a suitable guard strategy when matrices may foul the inlet. Establish adequate equilibration after changing solvent composition and monitor peak shape, retention, backpressure and resolution during operation. This is an analytical HPLC separation column, not a guard cartridge or a UHPLC sub-2 µm column.
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