Agilent, 600123, Obsolete. No replacement recommendation. T7 RNA Polymerase
Catalog Number: 600123
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Category
Pack Size10 units / pack
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| Concentration | 50 U/µL |
|---|---|
| Incubation Time | Incubate 30 minutes at 37 °C |
| Reaction Conditions | 40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 10 units enzyme in a 25 µL volume. Incubate 30 minutes at 37°C |
| Reactions per Kit | 5000 units |
| Storage Buffer | 20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50 % glycerol (v/v) |
| Unit Definition | One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C |
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