Agilent, 783975-906, AdvanceBio RP-mAb SB-C8, 4.6 x 150 mm, 3.5 µm

AdvanceBio RP-mAb SB-C8, 4.6 x 150 mm, 3.5 µm is designed for reversed-phase LC and LC–MS analysis of intact monoclonal antibodies, antibody subunits and proteins. This AdvanceBio RP-mAb SB-C8 column measures 4.6 mm in internal diameter and 150 mm in length and contains 3.5 µm superficially porous particles with a wide 450 Å pore size. The SB-C8 bonded phase provides reversed-phase retention while the wide pores improve access for larger proteins and antibody-derived analytes. StableBond chemistry supports separations at low pH, a common operating condition for protein reversed-phase methods. The operating range is pH 1–8, the maximum temperature is 90 °C and the pressure rating is 600 bar. Stainless-steel hardware supplies a conventional analytical LC connection and protects the packed bed during high-pressure operation. The column ships in acetonitrile/water and is supplied as one column. It is appropriate for biopharmaceutical characterization where differences in antibody or subunit hydrophobicity are separated chromatographically, including method development that couples reversed-phase chromatography to mass spectrometry. Its 450 Å pores and 3.5 µm superficially porous design distinguish it from small-molecule C8 columns having substantially narrower pores.