Agilent, 820750-921, AdvanceBio Oligonucleotide, 4.6 mm, fast guard, 2.7 µm

The Agilent AdvanceBio Oligonucleotide Fast Guard Column protects an analytical ion-pair reversed-phase LC separation by retaining particulate material and strongly retained contaminants before they reach the analytical bed. This HPH-C18 guard uses superficially porous 2.7 µm particles with 120 Å pores in a stainless-steel format measuring 5 mm in length and 4.6 mm internal diameter. The guard is supplied as a three-pack and is intended for oligonucleotide workflows, rather than standalone analytical resolution. Match the guard chemistry, bore, fittings and ion-pairing mobile phase to the analytical method. Install upstream of the separation column, maintain the indicated flow direction, and monitor backpressure and peak shape for signs of fouling. The documented operating limits are 600 bar, pH 3–11 and 65 °C; the complete assembled system may have lower limits.