| Brand | ZORBAX |
|---|---|
| Carbon Load | 0 % |
| Endcapped | No |
| Guard Column | No |
| Hardware | Conventional |
| Inner Diameter (ID) | 4.6 mm |
| LC Platform | HPLC |
| Length | 250 mm |
| Molecular Weight Lower Limit | 0 Da |
| Molecular Weight Upper Limit | 3000 Da |
| Particle Size | 5 µm |
| Particle Type | Fully Porous |
| Phase | Original SIL |
| Pore Size | 70 Å |
| Pressure Rating | 400 bar |
| Separation Mode | Normal Phase |
| UNSPSC Code | 41115711 |
| pH Range | 0.8-8 |
Answer:Normal-phase HPLC on an unbonded silica stationary phase.
Answer:4.6 mm internal diameter × 250 mm length with 5 µm silica particles.
Answer:No. Its polar bare-silica surface produces different retention and mobile-phase requirements.
The Agilent ZORBAX Original SIL column is a bare-silica stationary phase for normal-phase liquid chromatography. This analytical column measures 4.6 mm in internal diameter and 250 mm in length, with 5 µm fully porous silica particles and a specified pressure rating of 400 bar. Its polar surface provides retention and selectivity for compound classes that interact differently with silica under suitable normal-phase mobile-phase conditions. The configuration is useful for method development involving positional isomers, structurally related polar analytes and separations that are not readily achieved with reversed-phase chemistry. Because the phase is non-endcapped silica rather than bonded C18 material, solvent selection, water content and sample-solvent strength can substantially influence retention and peak shape. Condition the column with compatible solvents and maintain clean, particulate-free mobile phases. This is a packed HPLC analytical column, not a guard cartridge or GC capillary column. Method suitability should be established with reference standards.
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