Agilent, A3002300X039, Pursuit 200Å C18, 3.9 x 300 mm, 10 µm

The Agilent Pursuit C18 analytical column uses fully porous 10 µm silica particles with 200 Å pores and endcapped octadecyl bonding for reversed-phase HPLC. Its 3.9 mm internal diameter and 300 mm bed length provide a long-format separation path for methods requiring C18 hydrophobic retention of small organic molecules and selected biomolecular analytes. The 200 Å pore architecture supports access to the bonded phase by molecules that require more open pores than conventional small-pore packing. This conventional-hardware column is rated for pH 2–8, with listed limits of 400 bar and 60 °C. Method transfer should account for its 10 µm particles and 300 mm length rather than assuming performance identical to shorter 5 µm formats.