Agilent, CP29289, Chromspher 5 B, S150 x 4.6 Col.

The Agilent ChromSpher 5 B HPLC Column is a packed analytical liquid chromatography column in a 150 mm length and 4.6 mm internal diameter configuration. Its nominal 5 µm packing is intended for chromatographic separation work where a ChromSpher 5 B stationary phase is required by an established analytical method. The assembled column format provides a complete chromatographic unit for integration into an HPLC flow path with suitable end fittings, rather than a replacement cartridge that must be installed in a holder. Use the specified phase when reproducing an existing procedure, validating an instrument transfer or replacing a worn column without changing the separation chemistry. Before installation, confirm the prescribed mobile phase, flow direction, column-pressure limit and detector arrangement for the analytical method. Flush and equilibrate the column as required by the stationary-phase instructions, and evaluate retention, plate count and peak symmetry with appropriate reference analytes. Filter mobile phases and samples to limit particulate blockage at the inlet frit. Do not substitute ChromSpher 5 B for an unrelated C18, C8 or silica column solely because their dimensions match; chromatographic selectivity depends on the bonded-phase chemistry.