Agilent, GKE-5002, AdvanceBio Endoglycosidase H
AdvanceBio Endoglycosidase H
Catalog Number: GKE-5002
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| Application | Analysis of biosynthesis of glycoproteins |
|---|---|
| Concentration | ≥0.15 U/mL |
| Enzyme Applications | Selective release of high mannose N-linked oligosaccharides from glycopeptides/ glycoproteins. The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation. |
| Enzyme Formulation | 50 mM sodium phosphate pH 7.5, 25 mM EDTA, and 0.05% sodium azide |
| Enzyme Source | Recombinant gene from Streptomyces plicatus, expressed in E. coli. Enzyme is also known as Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 µmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5. |
| Unit | 30 mU |
| Volume | 200 µL |
| pH Optimum | 5.5 |
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