Agilent, GKE-5003, PNGase F, Chryseobacterium, native

Name: PNGase F, Chryseobacterium, native
Brand: Agilent
SKU: GKE-5003

PNGase F (native, Elizabethkingia mening

Catalog Number: GKE-5003

Pricing

Application	Structural analysis of O-glycan
Concentration	≥2 U/mL
Enzyme Applications\|MultiLine	PNGaseF is used to release intact N-linked glycans from glycopeptides and glycoproteins\|preparation of deglycosylated proteins for molecular weight estimation or crystallography studies\|structure-function studies of N-glycosylated glycoproteins\|
Enzyme Formulation	52 mM Tris-HCl, 36 mM NaCl, 9 mM sodium acetate, 5 mM EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation
Enzyme Source	Native, purified from Elizabethkingia meningoseptica. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum
Enzyme Specificity	PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.
Enzyme Unit Definition	One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37°C

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