Agilent, GKE-5006A-05, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
AdvanceBio N-Glycanase, PNGaseF, 5x100mU
Catalog Number: GKE-5006A-05
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Category
Pack Size5 vials
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| Concentration | ≥2.5 U/mL |
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| Enzyme Applications|MultiLine | Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies|Release of intact N-linked glycans from glycopeptides and glycoproteins|Structure-function studies of N-glycosylated glycoproteins| |
| Enzyme Formulation | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1]. Enzyme is also known asPNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | ~35,000 daltons |
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| Unit | 500 mU |
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| Volume | 40 µL |
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| pH Optimum | 8.6 |
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| pH Range | 7.5-9.5 |
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