Agilent, GKE-5006B, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

Name: AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Brand: Agilent
SKU: GKE-5006B

AdvanceBio N-Glycanase, PNGaseF, 200mU

Catalog Number: GKE-5006B

Pricing

Send an RFQ.

General

Concentration	≥2.5 U/mL
Enzyme Applications\|MultiLine	Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies\|Release of intact N-linked glycans from glycopeptides and glycoproteins\|Structure and function studies of N-glycosylated glycoproteins\|
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.

FAQs

Question: What bond does this enzyme cleave?

Answer:It cleaves the connection between an N-linked glycan's innermost GlcNAc and the peptide-bound asparagine.

Question: What quantity is supplied?

Answer:The package supplies 80 µL at ≥2.5 U/mL, totaling 200 mU.

Question: Which reagents accompany the enzyme?

Answer:The package includes reaction buffer, denaturation solution, detergent solution and Tris reaction buffer for MS workflows.

Application Note

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL is designed for enzymatic release of intact N-linked glycans from glycoproteins and glycopeptides for glycan profiling and protein characterization. This AdvanceBio N-Glycanase preparation contains recombinant peptide-N-glycosidase F (PNGase F), an enzyme that cleaves the linkage between the innermost N-acetylglucosamine of an N-linked glycan and its peptide-bound asparagine. The enzyme releases complex, hybrid and high-mannose N-glycans, except certain structures with α(1–3) core fucosylation. The preparation is endoglycosidase F-free and is derived from a recombinant gene expressed in Escherichia coli. This format supplies 80 µL at a concentration of at least 2.5 U/mL, equivalent to 200 mU per package. It includes 5× N-glycanase reaction buffer, denaturation solution, detergent solution and a 5× Tris reaction buffer for mass-spectrometry applications. The formulation contains 20 mM Tris-HCl, 50 mM NaCl and 1 mM EDTA at pH 7.5. The enzyme's optimal pH is 8.6 and its applicable pH range is 7.5–9.5. These components support preparation of deglycosylated proteins, assessment of N-linked glycan composition and structure–function experiments, including sample preparation before downstream glycan analysis.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.