Agilent, GKE-5006B-1, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
AdvanceBio N-Glycanase, PNGaseF, 5x200mU
Catalog Number: GKE-5006B-1
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Category
Pack Size10 units / pack
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| Concentration | ≥2.5 U/mL |
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| Enzyme Applications|MultiLine | Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies|Release of intact N-linked glycans from glycopeptides and glycoproteins|Structure-function studies of N-glycosylated glycoproteins| |
| Enzyme Formulation | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
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| Molecular Weight | ~35,000 daltons |
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| Unit | 1000 mU |
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| Volume | 80 µL |
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| pH Optimum | 8.6 |
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| pH Range | 7.5-9.5 |
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