Agilent, GKE-5020D, AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL
AdvanceBio N-Glycanase-ULTRA (PNGase F,
Catalog Number: GKE-5020D
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Category
Pack Size10 units / pack
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| Concentration | ≥10 U/mL |
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| Enzyme Applications | To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored. |
| Enzyme Formulation | 20 mM Tris HCl pH 7.5, 50 mM NaCl |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions. |
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| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
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| Molecular Weight | ~35,000 Daltons |
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| Unit | 1 U |
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| Volume | 100 µL |
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| pH Optimum | 8.6 |
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| pH Range | 7.5-9.5 |
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