Agilent, GKK-404, 2-AB Labeling Kit

Agilent Signal 2-AB Labeling Kit attaches a fluorescent 2-aminobenzamide tag to the reducing end of purified glycans by reductive amination. The resulting labeled glycans can be separated and measured in glycan profiling workflows using fluorescence-sensitive liquid chromatography. The kit supports up to 36 individual samples, arranged as two batches of 18, and is appropriate for purified glycan mixtures possessing free reducing ends. Its labeling reagents are distinct from the downstream cleanup materials; GlycoClean S cartridges and suitable solvents are needed separately. Reaction preparation, temperature and incubation time influence labeling consistency, so the complete method should be maintained across comparative studies. Samples should be handled in a manner that protects labile glycan features and minimizes contamination before injection. This formulation is the original Signal 2-AB kit; the newer 2-AB-plus kit is a different configuration with labeling and reducing reagents supplied in solution.