Agilent, GKK-804, 2-AB-plus Labeling Kit

The Agilent 2-AB-plus Labeling Kit supports fluorescent derivatization of purified glycans and glycan pools that have free reducing termini. Labeling uses 2-aminobenzamide (2-AB) and a reductant in solution, converting eligible glycans into fluorescent derivatives for chromatographic profiling and subsequent detection. The kit provides prepared labeling solutions rather than solid reagents requiring complete initial dissolution, simplifying batch preparation and allowing repeated use under suitable storage conditions. It contains 2-AB solution and reductant solution and can process up to 48 samples. Labeling is commonly integrated into released N-glycan workflows for characterization of glycosylated proteins, including therapeutic antibodies. Sample cleanup and dedicated equipment, including a heating block and suitable HPLC-grade solvents, are separate method requirements. The kit does not release glycans from proteins or perform the final chromatographic separation; it handles the fluorescence-labeling stage.