Agilent, GKSB-005, AdvanceBio 2-AB Human IgG N-glycan Library
AdvanceBio 2-AB Human IgG N-Glycan Libra
Catalog Number: GKSB-005
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| Label | 2-AB |
|---|---|
| Unit | 200 pmol |
Answer:Yes. The N-glycan reducing termini carry a 2-aminobenzamide (2-AB) label for fluorescence detection.
Answer:The vial contains 200 pmol of the 2-AB-labeled human IgG N-glycan library.
Answer:No. It is a pre-labeled glycan reference mixture; a separate labeling reagent kit is needed for unlabeled sample glycans.
The Agilent AdvanceBio 2-AB Human IgG N-Glycan Library contains fluorescently labeled oligosaccharides corresponding to complex biantennary N-glycans associated with normal human IgG. The glycan reducing termini are derivatized with 2-aminobenzamide (2-AB), allowing fluorescence-based detection during glycan profiling. The supplied quantity is 200 pmol per vial. This library provides a defined glycan mixture for method development, chromatographic peak-pattern comparison, and evaluation of workflows involving released, labeled N-glycans. It is particularly relevant to biopharmaceutical glycosylation studies, where glycan distributions are investigated using appropriate chromatography and detection conditions. The labeled library is a reference mixture, not a reagent for releasing glycans from an antibody or for derivatizing an unlabeled sample. Its value lies in the combination of a characteristic human IgG glycan profile and a consistent fluorescent label. Retention behavior depends on the chosen analytical column and separation method.
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