Agilent, GKX-5003, β-N-Acetylhexosaminidase (jack bean)
B-N-Acetylhexosaminidase (jack bean)
Catalog Number: GKX-5003
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| Concentration | 50 U/mL |
|---|---|
| Enzyme Applications | Useful for the structural analysis of isolated glycans and glycoconjugates. pH optimum/range: For glucosaminide 5.0 - 6.0, For galactosaminide 3.5 - 4.0 |
| Enzyme Formulation | 20mM sodium citrate phosphate (pH 6.0) |
| Enzyme Source | Jack bean. |
| Enzyme Specificity | The enzyme exhibits a broad specificity, cleaving nonreducing terminal ß(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß N-acetylhexosaminidase from S. pneumoniae (GK80050). We also offer this enzyme at a higher concentration - see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting ß(1-4)-linked GlcNAc residues in complex N-glycans. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C. |
| Molecular Weight | 100 - 110 kDa |
| Unit | 5 U |
| Volume | 100 µL |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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