Agilent, GKX-5006, Obsolete. No replacement recommendation. α(1-2,3,4,6)-Fucosidase (bovine kidney) (formerly ProZyme)
Catalog Number: GKX-5006
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| Enzyme Applications | Bovine derived a-fucosidase has been extensively used in the sequence analysis of N-glycans. It has also been used for analysis and modification of glycoconjugates including blood group oligosaccharides and glycolipids. |
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| Enzyme Formulation | Lyophilized from 20 mM sodium citrate phosphate, 0.25 mg/ml BSA (pH 6.0) |
| Enzyme Source | Bovine kidney. |
| Enzyme Specificity | The enzyme has broad substrate specificity, cleaving a(1-2,3,4,6)-linked fucose from N- and O-glycans. It cleaves a(1-6) linked fucose on the trimannosyl core of N-linked oligosaccharides more efficiently than other a-fucose linkages. The fine specificity of the enzyme is complicated since the aglycon portion of the substrate significantly influences the substrate kinetics. The rate of cleavage is lower with increasing oligosaccharide size and complexity. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP-α-fucopyranoside per minute at pH 6.0 and 37°C. |
| Molecular Weight | 210-220 kD |
| Unit | 500 mU |
| pH Optimum | 6 |
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