Agilent, GKX-5009, Obsolete. No replacement recommendation.See GK80075 for replacement enzyme. α(1-2)-Mannosidase (aspergillus saitoi) (formerly ProZyme)
Catalog Number: GKX-5009
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| Enzyme Formulation | Lyophilized from water containing 250 µg/ml bovine serum albumin. |
|---|---|
| Enzyme Source | Aspergillus saitoi. |
| Enzyme Specificity | The enzyme specifically cleaves a(1-2)-linked mannose from the nonreducing terminus of glycans. As such it is extremely valuable in identifying this type of linkage and, in particular, the presence of high mannose-type N-glycans that are digested to the Man-5 structure. |
| Enzyme Unit Definition | One Unit is defined as the amount of enzyme that will release 1 µmole of mannose from methyl-2-O-α-D-mannopyranosyl-α-D-mannopyranoside per minute at pH 5.0 and 37°C. |
| Molecular Weight | ~51 kD |
| Unit | 2 mU |
| pH Optimum | 5 |
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