Agilent, GKX-5023, β-(1-2,3,4,6)-Hexosaminidase, sequencing-grade (jack bean)
B-(1-2,3,4,6)-Hexosaminidase, Sequencing
Catalog Number: GKX-5023
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| Concentration | 300 U/mL |
|---|---|
| Enzyme Applications | The enzyme exhibits a broad specificity, cleaving non-reducing terminal ß(1-2,3,4, and 6)-linked-N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. It has proved very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß-N-Acetylhexosaminidase from S. Pneumoniae (GK80050). |
| Enzyme Formulation | 20mM sodium citrate phosphate (pH 6.0) |
| Enzyme Source | Jack bean. |
| Enzyme Unit Definition | One unit of ß-N-Acetylhexosaminidase is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from pNP-N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C. |
| Molecular Weight | ~67 kD (from literature) |
| Unit | 15 U |
| Volume | 50 µL |
| pH Range | 5.5-6 |
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