| Application|MultiLine | Flexible format|Nonselective, rapid release and recovery of intact N-glycans|Purified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or drying|RT labeling with InstantAB with reduced loss of sialic acid compared to reductive amination labeling chemistry|labeling efficiency >80%| |
|---|---|
| Count | 96 |
| Equipment & Reagents Provided by User|MultiLine | Acetonitrile (ACN, 100%, HPLC-grade)|AssayMAP Labware (AM200)|Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height|Formic acid (HPLC-grade)|Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)|Ultrapure, deionized water| |
| Kit Configuration | Sample Prep Kit |
| Label | InstantAB |
| Prep time (approx) | 3 hours |
| Technique | LC |
Answer:N-glycan digestion/release, fluorescent InstantAB labeling and removal of excess label using dedicated GlykoPrep modules.
Answer:It is configured for 96 samples, with digestion, labeling and cleanup modules included.
Answer:Yes. Required items include compatible labware, analytical-grade solvents, heating equipment and centrifugation; the package is not a complete instrument setup.
The Agilent GlykoPrep Rapid N-glycan Preparation Kit provides a cartridge-based workflow for releasing, labeling and cleaning up N-linked glycans from compatible glycoprotein samples before liquid chromatographic analysis. This 96-sample kit combines the GlykoPrep digestion module, InstantAB fluorescent labeling module and cleanup module, making it a complete sample-preparation set rather than a labeling-only refill. The process supports rapid, nonselective N-glycan release followed by room-temperature labeling and removal of excess dye; the purified fluorescent glycans are recovered in water for analysis without mandatory concentration or drying. An approximate three-hour preparation time is listed for the workflow. Additional laboratory materials, including solvents, incubation equipment, centrifugation and compatible labware, must be provided separately. Follow the recommended module sequence and sample input guidance to protect glycan recovery and consistency.
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