| Count | 96 |
|---|---|
| Equipment & Reagents Provided by User|MultiLine | Acetonitrile (ACN, 100%, HPLC-grade)|AssayMAP Labware (AM200)|Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height|Formic acid (HPLC-grade)|Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)|Ultrapure, deionized water| |
| Kit Configuration | Sample Prep Module |
| Label | InstantPC |
| Technique | LC & LC/MS |
Answer:InstantPC labeling is used for released N-glycans prior to fluorescence or LC/MS analysis.
Answer:The module is supplied in a 96-reaction format.
Answer:No. It supplies InstantPC labeling chemistry used in a broader GlykoPrep and chromatographic or mass-spectrometric workflow.
The Agilent GlykoPrep InstantPC Labeling Module provides fluorescent and mass-spectrometry-sensitive labeling chemistry for released N-glycans. The 96-reaction format is designed for glycan sample preparation ahead of HILIC chromatography, LC/MS and fluorescence-based analytical workflows. InstantPC labeling facilitates detection of low-abundance glycan species and supports both rapid screening and more detailed glycan characterization. The product is a labeling module within the GlykoPrep workflow rather than a complete LC/MS instrument or a stand-alone glycan profiling assay. Efficient preparation requires appropriate glycan release, labeling and cleanup steps, with reagents and laboratory equipment selected for the complete method. Agilent workflows accommodate short HILIC screening methods as well as higher-resolution separations. This format is relevant to biotherapeutic glycosylation assessment, method development and comparison of N-glycan distributions across samples.
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