| Count | 96 |
|---|---|
| Equipment & Reagents Provided by User|MultiLine | Centrifuge with a deep-well plate rotor (minimum 44 mm height)|Heater and incubation blocks (GS150)|Starter labware kit (AM200)| |
| Kit Configuration | Sample Prep Module |
| Protein Load Range | 1-50 µg |
Answer:It uses PNGase F to release intact N-linked glycans for downstream analysis.
Answer:The module is configured for 96 sample preparations.
Answer:The specified protein loading range is approximately 1–50 µg per sample.
Agilent GlykoPrep Digestion Module supports high-throughput preparation of released N-linked glycans from glycoprotein samples. The cartridge-based digestion workflow uses N-glycanase (PNGase F) to release intact N-glycans nonselectively before downstream cleanup, labeling, or chromatographic analysis in a broader GlykoPrep sample preparation process. The module is configured for 96 sample preparations and accepts approximately 1–50 µg of glycoprotein per sample. Its enzyme formulation is EDTA-free, helping maintain consistency within the intended digestion protocol. Compatible processing equipment and supporting GlykoPrep labware are required; the digestion module is not a standalone liquid chromatography instrument or a complete N-glycan profiling system. This format is designed for laboratories processing multiple glycoprotein samples in parallel for glycan characterization.
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