Agilent, GX24-401, AdvanceBio Gly-X 2-AB Express labeling module, 24-ct
AdvanceBio Gly-X 2-AB Express Labeling M
Catalog Number: GX24-401
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Category
| Application | Reductive amination 2-AB labeling chemistry without dry down |
|---|---|
| Count | 24 |
| Deglycosylation Efficiency | > 99 % |
| Equipment & Reagents Provided by User|MultiLine | Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sample|DI water, ~100 µl per sample|Gly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid)|Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks.|Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step.|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)| |
| Kit Configuration | Sample Prep Module |
| Label | 2-AB |
| Prep time (approx) | 130 min (24 samples) |
| Protein Load Range | 1-40 µg |
| Recovery | > 80 % |
| Stability | At least 1 month at -20°C, 5 days at 4–8°C |
| Technique | LC & LC/MS |
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