Agilent, GX24-IPC, AdvanceBio Gly-X N-glycan prep with InstantPC Kit, 24-ct
AdvanceBio Gly-X N-Glycan Prep with Inst
Catalog Number: GX24-IPC
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Category
Pack Size201 pc / pack
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| Application|MultiLine | <br />Optimized cleanup removes excess free dye, protein, and other interfering compounds|<br />Small molecule size for improved chromatographic peak resolution|Flexible, high-throughput format: process 1 to 24 samples|Note: The 24-count kit includes a 96-count cleanup plate. This plate can be stored at room temperature for future use. Gly-X InstantPC digestion and labeling reagents (GX24-201PC) are available separately for use with existing cleanup plates.|Other benefits include:|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.| |
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| Count | 24 |
| Equipment & Reagents Provided by User|MultiLine | 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional - VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)| |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Kit Configuration | Sample Prep Kit |
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| Label | InstantPC, Precision (well-to-well |
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| day-to-day) | <5% CV |
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| Prep time (approx) | 42 min (16 samples), 99 min (96 samples) |
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| Preservation of Sialylated Species | > 95 % |
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| Protein Load Range | 1-40 µg |
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| Recovery | > 95 % |
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| Stability | Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF |
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| Technique | LC & LC/MS |
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