Agilent, GX96-2AB, AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct

Name: AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct
Brand: Agilent
SKU: GX96-2AB

Gly-X 2-AB Express 96 sample kit

Catalog Number: GX96-2AB

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Category

Protein Labeling Kits

CollectionsAgilent HPLC and UHPLC Columns for Small Molecule Separations Agilent J&W GC Columns Agilent Biopharma HPLC Analysis

Description

AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct. Complete kit. utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-401 Gly-X 2-AB Express labeling module (96-ct), GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

Specifications

General

Application\|MultiLine	5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release\|Flexible, high-throughput format<br />Process 1 to 96 samples\|Optimized cleanup removes excess free dye, protein, and other interfering compounds.\|Reductive amination 2-AB labeling chemistry without dry down\|
Count	96
Deglycosylation Efficiency	> 99 %
Equipment & Reagents Provided by User\|MultiLine	96-well Thermocycler or two independent heat blocks\|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)\|DI water\|Thermocycler (Corning THERM-1001, 110V, THERM-1000, 230V)\|Vacuum manifold (Millipore MSVMHTS00-HTS)\|Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit - included free of charge with first Gly-X order, please contact us for details)\|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522)\|
Kit Configuration	Sample Prep Kit
Label	2-AB
Prep time (approx)	~2.5 hours
Preservation of Sialylated Species	> 99 %
Protein Load Range	1-40 µg
Recovery	> 80 %
Stability	At least 1 month at -20°C, 5 days at 4–8°C
Technique	LC & LC/MS

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