Agilent, PL1180-5301, AdvanceBio SEC 300Å HPLC Column, 7.8 x 300 mm, 2.7 µm

The Agilent AdvanceBio SEC 300 Å Analytical Column separates proteins and related biotherapeutic species primarily by their hydrodynamic size. Its 7.8 × 300 mm analytical bed contains fully porous 2.7 µm particles with a nominal 300 Å pore size. The column supports characterization of soluble protein monomers, higher-molecular-weight aggregates and lower-molecular-weight fragments, including monoclonal antibody preparations. Unlike ion-exchange or reversed-phase chromatography, size-exclusion methods aim to minimize analyte interaction with the stationary phase. Mobile-phase ionic strength and pH therefore require careful selection to limit secondary adsorption while maintaining protein stability. Use a filtered, compatible buffer, avoid sample overloading and monitor pressure to protect the packed bed. Equilibrate fully before establishing a retention profile and assess aggregate resolution with appropriate reference materials. A matching short SEC guard can protect the analytical bed from particulate and strongly retained contamination. The 300 mm column is the separation component, not the shorter 50 mm protective guard column.