CYTO-STAT tetraCHROME CD45-FITC/CD4-RD1/ CD8-ECD/CD3-PC5 and CYTO-STAT tetraCHROME CD45-FITC/CD56-RD1/CD19-ECD/CD3-PC5 Monoclonal Antibody Reagents are for use on the Cytomics FC 500, Navios, and Navios EX Flow Cytometers. The reagents are also used with the tetraCXP System for FC 500 Flow Cytometry System, Navios tetra Software for the Navios System, and Navios EX tetra Software for the Navios EX System.
Used alone or in combination with the automated systems, the reagents are intended "For In Vitro Diagnostic Use" and allow simultaneous identification and enumeration of total CD3+, total CD4+, total CD8+, dual CD3+CD4+, dual CD3+CD8+ and/or total CD3+, CD19+ and CD3-CD56+ lymphocyte percentages and absolute counts in whole blood by flow cytometry. The systems also provide the CD4/CD8 ratio when using CD45-FITC/CD4-RD1/ CD8-ECD/CD3-PC5. These reagents are indicated for use in the immunologic assessment of patients having or suspected of having immune deficiency.
| Target Species | Human |
|---|---|
| Specificity | CD45, CD4, CD8, CD3 |
| Fluorochrome | - |
| Regulatory Status | CE-IVD |
| Size | 50 Tests |
| Format | Liquid |
| Flow Product Line | cyto-stat |
| Volume | 0.5 mL |
Answer:CD45 is labeled with FITC, CD4 with PE, CD8 with ECD and CD3 with PC5.
Answer:A suitable four-color gating strategy separates CD45-positive leukocytes, CD3-positive T cells and their CD4-positive and CD8-positive subsets.
Answer:The liquid reagent is supplied as 0.5 mL for 50 tests and is designated CE-IVD.
The Beckman Coulter tetraCHROME CD45-FITC/CD4-PE/CD8-ECD/CD3-PC5 Antibody Cocktail combines four fluorescence-labeled monoclonal antibodies for multiparameter human T-cell immunophenotyping by flow cytometry. CD45-FITC supports leukocyte identification; CD3-PC5 identifies T lymphocytes; CD4-PE and CD8-ECD distinguish major T-cell subsets within the gated population. The ready-to-use liquid cocktail provides a consistent four-color reagent combination for laboratories following a validated staining, compensation and gating workflow. It is supplied as 0.5 mL for 50 tests and carries CE-IVD status in the specified market. Appropriate flow cytometer filters, controls and locally applicable diagnostic requirements remain important for reliable subset analysis. The cocktail is not a complete flow cytometry system or a standalone sample preparation kit.
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