Bio-Rad, 1300420, Bio-Gel HTP Hydroxyapatite

Bio-Rad Bio-Gel HTP Hydroxyapatite is a crystalline calcium phosphate chromatography medium supplied as 100 g of dry powder. It supports separations of proteins, antibodies and nucleic acids through interactions with the hydroxyapatite surface rather than conventional silica-based reversed-phase retention. One gram of dry medium hydrates to approximately 2–3 mL of gel, allowing the required settled volume to be planned before preparing a column. Hydration, equilibration and phosphate-buffer conditions influence sample binding and elution, so screen suitable gradients for the target biomolecule. The medium can be used in preparative fractionation when selectivity from calcium and phosphate surface interactions is useful. Control flow and bed packing gently to avoid channeling or a disturbed particle bed. Dry powder and hydrated resin have different handling and storage needs; protect unused powder from moisture and handle equilibrated resin under appropriate conditions. This general HTP product is not the separate DNA-grade HTP variant with smaller particles and modified double-stranded DNA selectivity.