Bio-Rad, 1560113, UNOsphere S Support
100 ml, strong cation exchange chromatography media, 80 μm particle size, UNOsphere S Support
Catalog Number: 1560113
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| Type of ion exchanger | Strong cation |
|---|---|
| Functional group | -SO3- |
| Total ionic capacity | 280 ± 30 µeq/ml |
| Dynamic binding capacity | 150 cm/hr: 60 mg IgG/ml 1,200 cm/hr: 30 mg IgG/ml (10% breakthrough capacity determined with 4.5 mg/ml human IgG) |
| Median particle size | 80 µm |
| Recommended maximum linear flow rate | 50–1,200 cm/hr UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure. |
| 1.0 M NaOH (20°C) | Up to 2,000 hr |
| 1.0 M HCl (20°C) | Up to 200 hr |
| pH stability range | pH 1–14 |
| Regeneration conditions | 1–2 M NaCl |
| Storage conditions | 20% Ethanol 0.1 N NaOH for 30 days |
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