Bio-Rad, 17007135EDU, Mammals, Insects, and Fungi DNA Barcoding Kit with Agarose and TAE

Name: Mammals, Insects, and Fungi DNA Barcoding Kit with Agarose and TAE
Brand: Bio-Rad
SKU: 17007135EDU

PCR and bioinformatics kit bundle, includes Mammals, Insects, and Fungi DNA Barcoding Kit (17007366EDU) and reagents for agarose gel electrophoresis of up to 16 DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation); for educational use only, Mammals, Insects, and Fungi DNA Barcoding Kit with Agarose and TAE

Catalog Number: 17007135EDU

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Category

Agarose

TagsLSG EDU - Bio-Education Science Education Classroom Educational Kits Kits Amplification>EDU_Kits DNA Barcoding Kits PCR and Real-Time PCR Kits

Description

Teach PCR, gel electrophoresis, and bioinformatics with this DNA barcoding lab activity in which students determine the species of a mammal or insect based on the DNA sequence of its cytochrome c oxidase I (COI) gene, or a fungus based on the DNA sequence of its internal transcribed spacer (ITS). Students first extract DNA from tissue, use PCR to amplify the DNA, and perform agarose gel electrophoresis to verify PCR products. Next, after receiving DNA sequencing results from a third party (sequencing services not included), students use bioinformatics to genetically identify the species using the sequence obtained. The unique COI or ITS sequence produced for a given sample is considered its DNA barcode.

This cost-saving bundle includes agarose and TAE electrophoresis buffer.

With this kit, you can teach your students how to:

• Perform DNA extraction, PCR amplification, and gel electrophoresis up to 16 DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation)

• Use DNA sequencing and bioinformatics to determine species identity

• Perform investigations in their area to examine biodiversity or see if marketplace substitution is occurring

• Contribute to global initiatives for barcoding life (Barcode of Life Database)

Learn more on the DNA Barcoding Kits product page.

Timeline

Finish all lab activities in three 45-minute class periods.

Product Contents

• DNA Extraction Module: reagents and consumable plastics for DNA extraction ( 12016408EDU )

• Mammals, Insects, and Fungi PCR Module: reagents and consumable plastics for PCR amplification, control DNA, DNA loading dye and stain, and molecular weight ruler ( 12016353EDU )

• Certified Molecular Biology Agarose, 25 g ( 1613100EDU )

• 50x Tris/Acetic Acid/EDTA (TAE), 100 ml ( 1660742EDU )

Related Products

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