Description
Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.
• For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers
• For separation by molecular weight use XT Sample and XT Tricine Running Buffers
• Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems
• 8-month shelf life
• Separation range is ~40–400 kD under denaturing conditions
• Can be loaded with a multichannel pipet
Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.
Packaging Options
Criterion XT Tris-Acetate Midi Protein Gels are available as 3–8% (separation range ~40–400 kD) or 7% (separation range ~35–250 kD) gels, with a selection of different well configurations.
Related Products
• Native Sample Buffer ( 1610738 )
• XT Sample Buffer, 4x, denaturing ( 1610791 )
• Tris/Glycine Native Running Buffer, 10x ( 1610734 )
• XT Tricine Running Buffer, 20x, denaturing ( 1610790 )
• XT Reducing Agent, 20x ( 1610792 )
• XT Tricine Buffer Kit ( 1610797 ), XT Tricine Running Buffer, XT Sample buffer, and XT Reducing Agent
Related Categories
• Protein Ladders and Standards (Markers) including unstained, prestained, and western standards
• Criterion Midi-Format Vertical Electrophoresis Cells , for 1 to 4 gels
• Criterion Dodeca Cell , for up to 12 gels
• 2-D Electrophoresis
• Western Blotting
• ChemiDoc™ and GelDoc™ Imaging Systems
Supporting Documents
• Criterion XT Precast Gel Instruction Manual
• Criterion Gel Application Guide
• Criterion XT Precast Gels Product Information Sheet
• Criterion Precast Gel System Brochure
• Electrophoresis Guide, Interactive PDF
Online Applications
• Protein Electrophoresis
• Western Blotting
