Bio-Rad, APO010A, Autophagy Assay Kit, Red

Autophagy Assay, Red Detection Kit allows for the detection and monitoring of in vitro development of autophagy in living cells. Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids and carbohydrates. Autophagy plays a critical role in maintaining homeostasis by preventing the accumulation of damaged organelles by disassembling unnecessary or dysfunctional cells and cellular components ( Mizushima et al . 2011 ). Autophagy occurs at low levels in the cell under normal conditions and can be rapidly upregulated during times of starvation or stress. Such degradation activities serve to provide nutrients (amino acids, nucleotides, fatty acids, etc.) and energy during periods of elevated bioenergetic demands ( Mizushima et al . 2011 , Levine et al . 2008 ). Another function of autophagy is to assist with the detection and destruction of intracellular pathogens (viruses, bacteria and parasites) ( Levine et al . 2011 ). Dysregulation of autophagy has been associated with many disease states including cancer, infection and degenerative diseases ( Levine et al . 2008 ). Autophagy is a dynamic process typically divided into three stages. During stage one, cytoplasmic components targeted for degradation are sequestered within a double-membrane phagopore (also called the isolation membrane). This results in the formation of a double-membrane vesicle called the autophagosome. During stage two, the autophagosome fuses with the lysosome to form the autolysosome. Degradation of the autophagosomal contents occurs during stage three ( Mizushima et al . 2011 , Hundeshagen et al . 2011 ).

Storage Information
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the Autophagy Probe, Red at -20°C. Once reconstituted in DMSO, the Autophagy Probe, Red stock should be stored at -20°C for 6 months, protected from light. Avoid repeated freezing and thawing.