Bio-Rad, BUF056B, TMB Core

TMB CORE is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP). TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB CORE produces a deep blue color during the enzymatic degradation of H 2 O 2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.

Storage Information
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product is photosensitive and should be protected from light.
Avoid exposure to heat and contamination with metal ions or peroxidase.
Store in bottles made of High Density Polyethylene (HDPE).