Bio-Rad, LNK014AP, Alk. Phos Conjugation Kit
Size: 3 Conjugations For 10µg Antibody, LYNX Rapid Alkaline Phosphatase Antibody Conjugation Kit
Catalog Number: LNK014AP
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Category
Pack Size3 vials
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| Regulatory | For research purposes only |
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| Guarantee | 12 months from date of despatch |
| Product Type | Conjugation Kit |
| Specificity | Alk. Phos Conjugation Kit |
| Reagents In The Kit | LNK012AP: 3 Vials of 100ug LYNX lyophilized AP mix 1 Vial LYNX Modifier reagent 1 Vial LYNX Quencher reagent |
| Preparing The Antibody | LNK012AP The following buffer solutions are recommended for preparing the antibody: 10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate*) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated. If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals . Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency. The molar ratio of antibody: AP should be 1:1, i.e. 100ug antibody to every 100ug AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 100ul and a maximum antibody amount of 100ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. * Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase. |
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| Licensed Use | These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: [email protected] |
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