Description
Use the Bio-Plex Pro cell signaling Akt 8-plex panel, an all-in-one premixed multiplex kit, for detecting the following phosphorylated analytes from cell lysates derived from cell culture or tissue samples:
• Akt (Ser 473 )
• BAD (Ser 136 )
• GSK-3α/β (Ser 21 /Ser 9 )
• IRS-1 (Ser 636 /Ser 639 )
• mTOR (Ser 2248 )
• p70 S6 kinase (Thr 389 )
• PTEN (Ser 380 )
• S6 ribosomal protein (Ser 235 /Ser 236 )
This assay panel measures the relative concentration of the phosphorylated protein.
Benefits
• All-in-one premixed kit
• Exceptional sensitivity (0.15–0.3 µg/well) and broad dynamic range
• Get started right away with the included assay quick guide
• Simplified assay processing with magnetic bead–based workflow to enable automation
Kit Components
• Coupled 6.5 µm magnetic beads
• Detection antibodies
• Cell lysate
• Cell lysis buffer
• Cell lysis factor QG
• Cell wash buffer
• Bead resuspension buffer
• Detection antibody diluent
• Wash buffer
• Streptavidin-PE
• 96-well flat bottom plate
• Sealing tape
Other Available Cell Signaling Assays
Browse our latest list of available phosphoprotein, total target, and housekeeping protein assays.
Other Assay Formats Available
Premixed all-in-one kits or custom Bio-Plex ® x-Plex™ assays.
Instrument Compatibility
Compatible with these readers and wash stations:
• Bio-Plex ® 200 or Luminex 200 system
• Bio-Plex ® 3D or Luminex FLEXMAP 3D system
• Bio-Plex ® MAGPIX™ or Luminex MAGPIX system
• Bio-Plex Pro™ wash stations
More Information
To learn more about performance characteristics or how to set up your cell signaling assays for optimal performance and sensitivity, refer to the documents listed below:
• Product information sheet
• Instruction manual
• Quick guide
