Bio-Rad, MCA2493, ALIX antibody | 3A9

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting ( Kim et al. 2005 ). ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 ( Mahul-Mellier et al. 2006 ) but has subsequently shown to be involved in endocytosis ( Matsuo et al. 2004 ), viral budding ( Irie et al. 2008 ) and actin cytoskeletal assembly ( Pan et al. 2006 ). Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting ( Sun et al. 2016 ) and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus ( Romancino et al. 2013 ).

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.