Description
PhoX, also known as (3,5-bis(((2,5-dioxopyrrolidin-1-yl)oxy) carbonyl)phenyl)phosphonic acid It is a crosslinker used to study protein structures and interactions by means of Cross-Linking Mass Spectrometry (XL-MS). PhoX is a novel and unique trifunctional crosslinker. Two N-hydroxysuccinimide (NHS) ester functions react rapidly and irreversible with amino functions in proteins, mainly neighboring lysine groups. The stable phosphonic acid function allows for the enrichment of mono- and crosslinked peptides by using the well-established immobilized metal affinity chromatography (IMAC). Overview of the available crosslinkers is shown in the table below. DSAU DSPU DSBU PhoX Type NHS ester Urea derivate NHS ester Urea derivate NHS ester Urea derivate NHS ester Phosphonic acid Chain C2 C3 C4 Chain length ~7.7 Å ~10.1 Å ~12.5 Å ~ 5 Å Examples of applications can be found in the following application notes and publications Improve your XL-MS results using the 4D-Proteomics based caps-PASEF acquisition method and the innovative PhoX crosslinker Steigenberger B et al. Benefits of Collisional Cross Section Assisted Precursor Selection (caps-PASEF) for Cross-linking Mass Spectrometry, Mol Cell Proteomics, 2020, doi: 10.1074/mcp.RA120.002094, Online ahead of print. Steigenberger B et al. PhoX: An IMAC-Enrichable Cross-Linking Reagent, ACS Cent Sci. 2019 Sep 25;5(9):1514-1522. Liu F. et al. Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry, Nat Methods. 2015 Dec 12(12), 1179-84. Klykov, O. et al. Efficient and robust proteome-wide approaches for cross-linking mass spectrometry, Nat Protoc 2018 Dec 13, 2964–2990. The purity is greater than 95%. The product is supplied in 5 x 2 mg vials. Vials are aliquoted under argon and in a sealed foil envelop packed under argon with desiccant. It is recommended to store the product on arrival at -20 °C or less. Additional resources: Material Safety Data Sheet (MSDS)
