354218, Corning® DiIC12(3) Fluorescent Dye, 100 mg

Name: Corning® DiIC12(3) Fluorescent Dye, 100 mg
Brand: Corning
SKU: 354218

Catalog Number: 354218

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Category

Fluorescent Dyes

Pack Size1/pk

TagsFluorescent Dyes

CollectionsCorning Reusable Glass Collection | Durable Borosilicate Labware Corning Microcentrifuge Tubes (MCTs) – Reliable 1.5 mL & 2 mL Lab Tubes Corning Robotic Tips & Reservoirs for Automated Liquid Handling

Description

The fluorescent dyeDiIC12(3) can be used with Corning FluoroBlok Cell Permeable Supports and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays. DiIC12(3) is a lipophilic neuronal tracer that is commonly used for labeling neuronal projections as well as lipid bilayers in other cell types. Because DiIC12(3) exhibits low toxicity and minimal effects on cell viability, this dye can be used to pre-label cells for a variety of applications. Corning DiIC12(3) Fluorescent Dye can be used to fluorescently label viable cells for assays such as tumor cell invasion or endothelial cell migration. Moreover, cells are known to tolerate different fluorescent dyes for varying amounts of time in culture. While cells labeled with calcein AM should be exposed to the dye for less than eight hours, DiIC12(3) can be used for several days in culture without adversely affecting cells. Preparation and Storage Product is shipped on dry ice. Upon receipt, store immediately at -20°C. Stable for at least three months from date of shipment. Product Size: 500 µg Quality Purity: >95% Extinction Coefficient: >150,000 M-1cm-1

Specifications

General

Product Number	354218
Qty./Pk	1 / Pk
Qty./Cs	1 / Ea
Brand	Corning®
Size	100 mg

FAQs

Question: What types of experiments can use DiIC12(3) fluorescent dye?

Answer:It can label viable cells for migration, invasion, tubulogenesis and membrane or neuronal tracing studies.

Question: What quantity is supplied?

Answer:This configuration contains 100 mg of fluorescent dye.

Question: How should the dye be stored?

Answer:Store the product at -20°C after receipt.

Application Note

Corning® DiIC12(3) Fluorescent Dye is used to label viable cells and lipid membranes for fluorescence-based cell migration, invasion and tracing studies. DiIC12(3) is a lipophilic carbocyanine dye that partitions into cell membranes and can be used as a neuronal tracer or for labeling other cell types before functional assays. Corning identifies applications including tumor-cell invasion, endothelial-cell migration and endothelial tubulogenesis with FluoroBlok permeable supports and related insert systems. The dye has low toxicity under appropriate conditions and can remain useful for cell labeling over several days, making it suitable for experiments that require longer tracking than short-lived viability dyes. This configuration is supplied as 100 mg with stated purity above 95%. The material is shipped frozen and should be stored at -20°C after receipt.

Ordering

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