354350, Corning® ITS Premix Universal Culture Supplement, 20 mL, 1/Pack

Corning® ITS Universal Culture Supplement, 20 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 20 mL (20L equivalent), 1/pack The ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin (5ug/ mL), Transferrin (5ug/ mL), and Selenious Acid (5ng/ mL) Formulation Corning ITS Premix -Lyophilized from a water solution; reconstitute in 20 mL (cat. no. 354350) or 5 mL (cat. no. 354351) dH2O (stock solution) Quality Tested for ability to promote proliferation of HeLa cells in serum-free medium Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma Preparation and Storage Corning ITS Premix - Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Avoid multiple freeze/thaws. Tip Corning ITS Premix - Recommended dilution: 1:1000 (dilute stock solution in serum-free medium)