356043, Corning® Interleukin-2 (IL-2), Human Recombinant, 50,000 BRMP Units, 1/Pack

Corning® Interleukin-2 (IL-2), human recombinant, 5000 BRMP units, promotes long-term proliferation of activated T-cells. Also known as T-cell growth factor, Interleukin-2 (IL-2) plays a central role in cell-mediated immune response. 1/pack, formerly Cat. #40043B These cytokines have been used to promote long-term proliferation in cell culture and to induce cell-mediated immune responses in a variety of cell types. Formulation Frozen in 55 mM sodium phosphate buffer containing 53 mM sucrose and 0.4% HSA Preparation and Storage Stable for at least three months at -70°C; =one month solubilized at -70°C Tip Recommended concentration: 10 BRMP units/ mL of medium depending on cell type Quality Purity: >96% by SDS-PAGE Tested for ability to stimulate proliferation of the mouse T-cell line CTLL Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma Tested for endotoxin (LAL assay) Source Recombinant, E. coli Molecular Weight 15.5 kD References Hatakeyama, M. and Taniguchi, T., Interleukin-2 in Peptide Growth Factors and Their Receptors I, Springer-Verlag, NY (1990) Kashima, N., et al., Nature 313:402 (1985) Leonard, W.J., et al., Proc. Natl. Acad. Sci. USA 80:6957 (1983) Mori, H., et al., Int. Immunol. 3:149 (1991) Morgan, D.A., et al., Science 193:1007 (1976) Smith, K.A., Science 240:1169 (1988) Meidema, F. and Melief, J.M., Immunol. Today 6:258 (1985) Triachiesi, T., et al., J. Exp. Med. 16:1147 (1984) Grimm, E.A., et al., J. Exp. Med. 155:1823 (1982) Storage Shelf life: >= 3 months from date of shipment at -70°C or >= one month solubilized at -70°C