Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunofluorescence (Frozen): 1:100
Immunofluorescence (Immunocytochemistry): 1:3000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ALCAM (F3V8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALCAM protein. Nuclear background is observed in cultured mouse cells by immunofluorescence.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly566 of human ALCAM protein.
Background
Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 or MEMCAM, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed in various cell types, including leukocytes, fibroblasts, epithelial cells, and neurons. ALCAM functions primarily as a cell adhesion molecule, mediating both homotypic (ALCAM-ALCAM) and heterotypic interactions with other cell surface receptors, most notably CD6 (ALCAM-CD6). These interactions, and others, play critical roles in various physiological and pathological processes, including hematopoiesis, neuronal development and function, T cell activation and proliferation, immune response, angiogenesis, and tumor progression and metastasis (1-5). Aberrant ALCAM expression is frequently observed in various human tumors, including melanoma, prostate cancer, breast cancer, colorectal carcinoma, bladder cancer, and pancreatic adenocarcinoma. High ALCAM expression correlates with poor prognosis and increased metastatic potential in many of these tumor types (5). ALCAM can mediate tumor-endothelial cell interactions, facilitating tumor cell dissemination and extravasation to distant sites such as the brain, bone, and liver. It has been implicated in promoting tumor cell invasion, migration, and survival in the tumor microenvironment and may contribute to chemotherapy drug resistance in some cancers (5).
Alternate Names
activated leucocyte cell adhesion molecule; Activated leukocyte cell adhesion molecule; ALCAM; CD166; CD166 antigen; FLJ38514; MEMD; MGC71733
