CST, 10691S, ADA2B (F6G5T) Rabbit Monoclonal Antibody

Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ADA2B (F6G5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADA2B protein. This antibody does not cross-react with ADA2A protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp172 of human ADA2B protein.
Background
ADA2A and ADA2B are human homologs of the yeast SAGA complex member Ada2. While highly related, ADA2A and B appear functionally distinct. ADA2A is incorporated into PCAF-containing complexes, while ADA2B is recruited to SAGA complexes with GCN5 and Brg1 (1-3). ADA2 proteins form a trimer with GCN5L and ADA3, and enhance GCN5L acetyltransferase activity (4,5). The C-terminal domain of ADA2A and ADA2B determines incorporation into their various complexes, resulting in specific targeting toward histone H3 or H4 (6).
Alternate Names
ADA2-beta; ADA2-like protein beta; ADA2(beta); ADA2B; MGC21874; TAD2B; TADA2B; Transcriptional adapter 2-beta; transcriptional adaptor 2 (ADA2 homolog, yeast)-beta; transcriptional adaptor 2B