Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ADRM1 (D9Z1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADRM1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ADRM1 protein.
Background
Currently, there are five ubiquitin receptors associated with the proteasome: two proteasome subunits, Rpn10/S5a/PSMD4 and Rpn13/ADRM1 (Adhesion-regulating molecule 1), and three families of shuttling factors, Rad23, Dsk2, and Ddi1. ADRM1 is a ubiquitin receptor of the proteasome (1,2) that binds ubiquitin via a pleckstrin homology domain known as the pleckstrin-like receptor for ubiquitin (Pru) domain. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37 (3-5), perhaps serving as a mechanism to accelerate ubiquitin chain disassembly. A murine knockout results in defective gametogenesis, thus highlighting a physiologic role for endogenous ADRM1 in mammalian development (6).
Alternate Names
110 kDa cell membrane glycoprotein; adhesion regulating molecule 1; Adhesion-regulating molecule 1; ADRM1; ARM-1; ARM1; GP110; hRpn13; M(r) 110,000 surface antigen; MGC29536; Proteasomal ubiquitin receptor ADRM1; Proteasome regulatory particle non-ATPase 13; proteasome ubiquitin receptor; regulatory particle non-ATPase 13; Rpn13; Rpn13 homolog
