Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ADAMTS1 (D5G4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADAMTS1 protein. This antibody does not cross-react with other ADAM or ADAMTS proteins.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg187 of human ADAMTS1 protein.
Background
A Disintegrin and Metalloprotease with Thrombospondin Motifs (ADAMTS) proteins comprise a large family of secreted zinc metalloproteases that play important roles in various processes, including organogenesis, hemostasis, and angiogenesis (1,2). ADAMTS proteases show structural similarity to ADAM proteases, but are further defined by the presence of repeated carboxy-terminal motifs homologous to the anti-angiogenic type 1 repeats of thrombospondin-1 (3). Functions ascribed to ADAMTS proteases include regulating the extracellular bioavailability of cytokines and growth factors (4, 5), regulating cell adhesion to the extracellular matrix (ECM), and remodeling of the ECM (6). ADAMTS1 has been shown to possess potent anti-angiogenic activity (2) and is reportedly dysregulated in a number of cancer subtypes (7). Functional studies in an ADAMTS1 knockout mouse model suggested that ADAMTS1 promotes metastatic invasion of breast carcinoma cells (8). These studies showed a reduced tumor burden in ADAMTS1 knockout mice, which was linked to increased cytotoxic immune cell invasion and reduced tumor cell survival (8).
Alternate Names
A disintegrin and metalloproteinase with thrombospondin motifs 1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAM-TS1; ADAMTS-1; ADAMTS1; ATS1; C3-C5; human metalloproteinase with thrombospondin type 1 motifs; KIAA1346; METH-1; METH1
