Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
5-HTR4 (D8O5K) Rabbit Monoclonal Antibody recognizes endogenous levels of total 5-HTR4 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala361 of human 5-HTR4 protein.
Background
Serotonin (5-hydroxytryptamine, 5-HT) is a central nervous system monoamine implicated in a number of physiological functions, including sleep, mood, appetite, anxiety, and neurovegetative control (1). Serotonin acts through the activation of G protein receptors called serotonin receptors (5-HTRs). Seven 5-HTR subclasses (5-HTR1-7) have been described to date (2). Serotonin receptor 4 (5-HTR4) couples to Gαs (3), with stimulation of the receptor leading to adenylyl cyclase activation, elevation of cyclic AMP levels and downstream PKA activation (4). The corresponding gene exhibits a complex structure, which includes a large number of splice variants that seem to share the same pharmacological properties (5). 5-HTR4 is one of several receptors implicated in cognition, depression, and the development of Alzheimer's disease (6-8).
Alternate Names
5-HT-4; 5-HT(4); 5-HT4; 5-HT4R; 5-hydroxytryptamine (serotonin) receptor 4; 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled; 5-hydroxytryptamine receptor 4; 5-hydroxytryptamine receptor 4 transcript variant b2; 5-hydroxytryptamine receptor 4 transcript variant b3; 5-hydroxytryptamine4 receptor; 5HT4R; 5HTR4; cardiac 5-HT4 receptor; htr4; serotonin 5-HT4 receptor; serotonin receptor 4; Serotonin receptor 5-HT4
