Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
APC11 (D1E7Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total APC11 protein. This antibody does not cross-react with either RBX1 or RBX2.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a full-length human recombinant APC11 protein.
Background
Eukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC/C function relies on multiple cofactors, including an APC/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1/FZR1. The CDC20/Cdh1 coactivator is responsible for recognition of APC/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9). Anaphase-promoting complex subunit 11 (APC11) harbors a RING-H2 motif, which is characterized by a series of non-tandem His and Cys residues responsible for the coordination of zinc cations. At the primary amino acid level, APC11 displays sequence similarity to RING-box proteins RBX1 and RBX2, which are the RING-H2 motif-containing subunits of SCF ubiquitin ligase complexes (10). A heterodimer complex containing APC11 and the cullin-like subunit, APC2, forms the catalytic core of the APC/C and is critical for the APC/C to catalyze ubiquitin chain elongation (4,11).
Alternate Names
ANAPC11; anaphase promoting complex subunit 11; anaphase promoting complex subunit 11 (yeast APC11 homolog); Anaphase-promoting complex subunit 11; APC11; APC11 anaphase promoting complex subunit 11 homolog; Apc11p; Cyclosome subunit 11; Hepatocellular carcinoma-associated RING finger protein; HSPC214; MGC882
