Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ANT2/SLC25A5 (E2B9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ANT2/SLC25A5 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly156 of human ANT2/SLC25A5 protein.
Background
Adenine nucleotide translocase 2 (ANT2/SLC25A5) is a member of the adenine nucleotide translocase family of mitochondrial inner membrane proteins that function differently in metabolic and apoptotic pathways (1). Research studies indicate that ANT2 expression in undifferentiated, proliferating cells correlates with the rate of glycolytic metabolism and may be an indicator of carcinogenesis (2). Suppression of ANT2/SLC25A5 expression by specific RNA interference in human breast cancer cells promotes apoptosis and inhibits tumor cell growth (2), which suggests a cytoprotective role of ANT2/SLC25A5 (1,3).
Alternate Names
2F1; AAC2; Adenine nucleotide translocator 2; adenine nucleotide translocator 2 (fibroblast); adenine nucleotide translocator), member 5; ADP,ATP carrier protein 2; ADP,ATP carrier protein, fibroblast isoform; ADP/ATP carrier protein; ADP/ATP translocase 2; ADP/ATP translocase 2, N-terminally processed; ADT2; ANT 2; ANT2; SLC25A5; solute carrier family 25 (mitochondrial carrier; Solute carrier family 25 member 5; T2; T3
