CST, 15020S, DAPT

Product Usage Information
DAPT is supplied as a lyophilized powder. For a 25 mM stock, reconstitute 5 mg in 462.43 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but are typically used at 10-50 μM for 12-48 hr, respectively.
Storage
Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
DAPT is a potent γ-secretase inhibitor. Research studies have demonstrated that DAPT inhibits production of total β-amyloid peptide (Aβ) and Aβ42 in human primary neuronal cultures with an IC of 115 nM and 200 nM, respectively (1). DAPT-induced inhibition of Notch1 signaling prevents cleavage formation of the Notch1 intracellular domain (NICD), resulting in down regulation of target gene transcription (2). DAPT treatment has been shown to induce apoptosis in Jurkat (2) and lung squamous cell carcinoma (3) cell lines.
Alternate Names
amyloid; beta-amyloid; gamma; GSI; nicastrin; notch; notch1; PEN2; Presenilin; secretase